Project on Type 2 diabetic and Hyperglycemic Pancreatic Islets

Introduction

Emma

Materials - The Data Set Description

Data set overview:

• 14481 different genes

• 63 samples

  • 9 with T2D

  • 54 controls

Descriptive statistics:

• Similar mean and median gene expression across groups

• Overall low mean expression levels

• Right-skewed distribution caused by some higher expressed genes

• The two groups show a similar distribution but NOT identical

• => Investigate significant difference of genes between the groups

Materials - Wrangling

Frederik

Methods - Median Expression Differences

• Goal: Identify genes with largest expression differences between T2D and control
• Approach:
  • Compute median expression per gene for T2D and control samples
  • Calculate absolute differences between groups per gene
• Visualization:
  • Plot 30 genes with the most different expressions
  • Bar plot and boxplots

Methods - p.values

Bolette

Results - Median

Lena

Results - p.value

Emma

Results - combined / correlation matrix

vælger 10 gener, correlation matrix

Bolette

Discussion / Conclusion

1) Difference in two methods

2) Sum up which genes are found by the analysis

3) does they support the litterature? uniprot…